CRISPR Research Volunteer

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Join now:

https://join.slack.com/t/futuretechfoundation/shared_invite/zt-2p4vtweqa-DNfYAGJMBseovqxu_0~VPQ

Role Overview:

We are seeking enthusiastic and dedicated volunteers to assist with an exciting project involving CRISPR-Cas9 technology to enhance osteogenic potential by targeting key genes like Runx2 and ALP. Volunteers will be an integral part of a cutting-edge research team focused on gene editing to promote bone formation and osteoblast differentiation in mice.

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Key Responsibilities:

CRISPR gRNA Design: Assist in designing and optimizing CRISPR guide RNAs for gene targeting, focusing on genes like Runx2 and ALP.

Cell Culture: Help with the preparation and maintenance of osteoblast precursor cell cultures for gene editing.

CRISPR-Cas9 Transfection: Aid in transfecting cells with CRISPR components (Cas9 and gRNAs) and assist with screening for successful edits.

Molecular Analysis: Perform PCR, RT-PCR, and qPCR to assess gene expression changes after CRISPR editing.

Data Collection & Analysis: Assist with data collection, including gene expression analysis, protein assays (such as ALP activity), and osteogenesis assays (e.g., mineralization).

Documentation: Maintain accurate and organized records of experiments and results.

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Qualifications:

Basic understanding of molecular biology and CRISPR technology.

Experience in cell culture or molecular biology techniques (PCR, gel electrophoresis, etc.) is a plus.

Enthusiasm for learning and contributing to genetic engineering research.

Ability to work independently and as part of a team.

Attention to detail and strong organizational skills.

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Benefits:

Gain hands-on experience in gene editing and CRISPR-Cas9 technology.

Contribute to meaningful research aimed at advancing osteogenic therapies.

Opportunity to develop lab and research skills in a professional environment.

Mentorship from experienced researchers in the field of genetic engineering and regenerative medicine.